2018缬沙坦预处理对乳鼠心肌细胞缺氧/复氧损伤的保护作用

8615043

　　【摘要】目的:探讨缬沙坦预处理对乳鼠心肌细胞缺氧/复氧损伤的保护作用及其机制。方法:常规方法培养心肌细胞，培养72小时后，分为三组：正常对照组、心肌细胞缺氧/复氧损伤组、缬沙坦预处理后心肌细胞缺氧/复氧损伤组。分别观察心肌细胞结构，测定心肌细胞存活率、乳酸脱氢酶(LDH)、丙二醛(MDA)及超氧化物歧化酶(SOD)水平。结果:与对照组比心肌细胞缺氧/复氧损伤组细胞存活率明显下降、培养液中LDH、MDA含量明显增高，SOD含量明显下降；与心肌细胞缺氧/复氧损伤组比，缬沙坦预处理组心肌细胞存活率明显提高，培养液中LDH、MDA含量明显下降、SOD含量明显上升。结论:缬沙坦通过抗氧化、抗脂质过氧化反应，清除氧自由基对乳鼠心肌细胞缺氧/复氧损伤有保护作用。
http://
　　【关键词】缬沙坦；心肌细胞；缺氧复氧；预处理
　　【中图分类号】R322.11;R541.4【文献标识码】A【文章编号】1008-6455（2011）10-0036-02Protective effects of Valsartan pretreatment on hypoxia/reoxygen injury in neonatal SD rat cardiomyocytes
　　Wang Liang1Dai Zhenhua2Chen Peng3
　　【Abstract】Objective:To observe protective action of Valsartan pretreatment on hypoxia/reoxygen injury in neonatal SD rat cardiomyocytes and to investigate the mechanism.Methods:To establish a model for primary culture of cardiomyocytes injuried by hypoxia/reoxygen. The morphology of cardiomyocytes was observed, and cardiomyocytes fraction surviving were measured, and the activity of lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD) were assayed.Results:Valsartan pretreatment on hypoxia/reoxygen injury in neonatal SD rat cardiomyocytes inhance cardiomyocytes fraction surviving (P